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Fig. 6. SFJD treated viral pneumonia in mice via the TLR4/MyD88/NF-κB immune signaling pathway. (A) Protein expression strip chart. (B−E) Protein expression of TLR4, MyD88, <t>TRAF6</t> and p-NF-κB in lung tissues. (F−G) ELISA for TNF-a and IL-6 expression in lung tissue (n = 6). Data were shown as the mean ± SD. #P < 0.05, ##P < 0.01, ###P < 0.001 vs control group and control + SFJD-H group; *P < 0.05, **P < 0.01, ***P < 0.001 vs model group.
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Fig. 6. SFJD treated viral pneumonia in mice via the TLR4/MyD88/NF-κB immune signaling pathway. (A) Protein expression strip chart. (B−E) Protein expression of TLR4, MyD88, <t>TRAF6</t> and p-NF-κB in lung tissues. (F−G) ELISA for TNF-a and IL-6 expression in lung tissue (n = 6). Data were shown as the mean ± SD. #P < 0.05, ##P < 0.01, ###P < 0.001 vs control group and control + SFJD-H group; *P < 0.05, **P < 0.01, ***P < 0.001 vs model group.
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Fig. 6. SFJD treated viral pneumonia in mice via the TLR4/MyD88/NF-κB immune signaling pathway. (A) Protein expression strip chart. (B−E) Protein expression of TLR4, MyD88, <t>TRAF6</t> and p-NF-κB in lung tissues. (F−G) ELISA for TNF-a and IL-6 expression in lung tissue (n = 6). Data were shown as the mean ± SD. #P < 0.05, ##P < 0.01, ###P < 0.001 vs control group and control + SFJD-H group; *P < 0.05, **P < 0.01, ***P < 0.001 vs model group.
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Fig. 6. SFJD treated viral pneumonia in mice via the TLR4/MyD88/NF-κB immune signaling pathway. (A) Protein expression strip chart. (B−E) Protein expression of TLR4, MyD88, <t>TRAF6</t> and p-NF-κB in lung tissues. (F−G) ELISA for TNF-a and IL-6 expression in lung tissue (n = 6). Data were shown as the mean ± SD. #P < 0.05, ##P < 0.01, ###P < 0.001 vs control group and control + SFJD-H group; *P < 0.05, **P < 0.01, ***P < 0.001 vs model group.
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Fig. 6. SFJD treated viral pneumonia in mice via the TLR4/MyD88/NF-κB immune signaling pathway. (A) Protein expression strip chart. (B−E) Protein expression of TLR4, MyD88, TRAF6 and p-NF-κB in lung tissues. (F−G) ELISA for TNF-a and IL-6 expression in lung tissue (n = 6). Data were shown as the mean ± SD. #P < 0.05, ##P < 0.01, ###P < 0.001 vs control group and control + SFJD-H group; *P < 0.05, **P < 0.01, ***P < 0.001 vs model group.

Journal: Chinese Herbal Medicines

Article Title: Exploring possible mechanisms of antiviral effect of Shufeng Jiedu Capsule on viral pneumonia: A comprehensive analysis

doi: 10.1016/j.chmed.2025.01.007

Figure Lengend Snippet: Fig. 6. SFJD treated viral pneumonia in mice via the TLR4/MyD88/NF-κB immune signaling pathway. (A) Protein expression strip chart. (B−E) Protein expression of TLR4, MyD88, TRAF6 and p-NF-κB in lung tissues. (F−G) ELISA for TNF-a and IL-6 expression in lung tissue (n = 6). Data were shown as the mean ± SD. #P < 0.05, ##P < 0.01, ###P < 0.001 vs control group and control + SFJD-H group; *P < 0.05, **P < 0.01, ***P < 0.001 vs model group.

Article Snippet: Anti-F4/80 antibody was provided by HUABIO (RT1212; Zhejiang, China); anti-TLR4 antibody, anti-MyD88 antibody and anti-NF-κB antibody were provided by Proteintech (10005089, 10022637, 66535; Wuhan, China); anti-NF-κB p65 antibody was provided by Abcam (ab76302; Cambridge, UK); antiTNF receptor associated factor (TRAF6) antibody was provided by Santa Cruz (sc-8409; Santa Cruz, USA,).

Techniques: Expressing, Stripping Membranes, Enzyme-linked Immunosorbent Assay, Control